FastQCFastQC Report
Sun 14 Apr 2019
SRR6577007_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577007_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614845
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11180.18183444607990631No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9720.1580886239621368No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9000.14637835552049702No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8750.1423122900893721No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8530.1387341525099822No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8370.13613187063406226No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC8130.1322284478201823No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8110.13190316258569235No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7980.12978880856150737No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7960.12946352332701738No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7820.12718652668558741No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7520.12230724816823751No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC7230.11759061226813262No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7100.11547625824394765No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6460.10506713074026788No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG6340.1031154193333279No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6190.10067578007465297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11450.059.6540571
ATCAACG14400.045.044243
CAACGCA14800.043.8268245
TCAACGC15350.042.5626874
AACGCAG15650.041.1461226
GGTATCA3950.039.3544961
GTACTAG1500.037.6849141
TATCAAC18700.036.6972852
ACGCAGA17850.035.8057447
GGTAATC1451.8189894E-1135.651418
CGCAGAG18350.034.8301128
AAGGGTA1651.8189894E-1234.183775
GCAGAGT19950.032.272289
CTAACGC1052.062381E-631.3351213
CCCTAGC650.00615025428.9247282
CTCTACG650.006155144428.9200239
GTATAAG1853.1832315E-1028.0090541
GTGTAAA856.723198E-427.7094941
AGAGTAC18300.027.34957310-11
TAGGCAT3100.027.2918825