FastQCFastQC Report
Sun 14 Apr 2019
SRR6577008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555618
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10540.18969867786860758No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10180.18321940613875No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9630.17332051877368984No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9550.17188068061149928No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8910.1603619753139747No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8570.15424266312466478No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8570.15424266312466478No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8110.14596359369206902No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7990.14380383644878317No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7730.13912436242166382No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC7140.12850555597550833No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6990.12580585942140102No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6720.12094640562400787No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT6630.11932658769154347No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG6540.11770676975907908No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6530.11752678998880525No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6260.11266733619141207No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6190.11140747779949535No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA5940.10690798354264981No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC5790.1042082869885425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10550.057.9085961
TCTAGTA250.005236802656.394062
GTACTAG807.8216544E-1152.869431
ATCAACG12500.047.7383773
CAACGCA13200.045.2067955
GGTATCA3050.043.1429981
TCAACGC14150.042.5037734
AACGCAG14450.041.9465076
GTATAAG853.9539918E-738.7018051
TTAGGTA2300.036.7721144
TATCAAC17300.036.1291432
ACGCAGA16400.036.0994537
ATAGGTA1850.035.5574294
CGCAGAG16750.035.3451358
CGGGCGT1950.033.7339636
CTTAGGT2550.033.1670043
GCAGAGT18200.032.529189
AGGTATA2650.031.9154196
GTACATG20600.031.710251
TACATGG20450.031.4832382
Produced by FastQC (version 0.11.2)