FastQCFastQC Report
Sun 14 Apr 2019
SRR6577008_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577008_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555618
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10310.18555914315230967No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9530.17152072107095162No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8660.15586248105712916No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8310.14956318909754543No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8230.14812335093535486No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8080.14542365438124755No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7740.13930434219193763No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7550.13588472655673503No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7500.1349848277053659No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7390.13300505023235387No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7230.13012537390797274No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7160.12886551551605602No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6890.12400606171866282No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG6190.11140747779949535No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6070.10924772055620949No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5980.10762790262374508No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5810.10456824652909014No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA5800.1043882667588163No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5750.10348836790744721No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5680.10222850951553046No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT5640.10150859043443518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9900.055.6270031
GGTATCA3100.047.0690041
CAACGCA12150.045.6390535
ATCAACG12200.045.0668223
AACGCAG13150.042.1684046
CGTTAAC450.001006212241.839111
TCAACGC13200.041.2966654
ACGCAGA14500.037.9182937
TATCAAC15650.035.732512
CGCAGAG15450.035.6123858
GTACTAG1651.8189894E-1234.2321
GTACATG20850.032.5080871
GCAGAGT16900.032.278629
TACATGG20650.031.8594952
CTTAGGT2850.029.6796343
ACATGGG22050.029.623553
TTAGGTA2700.029.5880324
TACTCCG1602.1318556E-929.370475
GTATTAA650.00610753328.9655381
GTACTGT2600.028.918626
Produced by FastQC (version 0.11.2)