FastQCFastQC Report
Sun 14 Apr 2019
SRR6577013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570888
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14040.24593265228906544No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9980.17481537534507643No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8710.15256933058673505No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7640.13382659996356552No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7350.12874679446756632No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7190.1259441431594288No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7170.12559381174591164No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7060.12366698897156711No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6990.12244082902425696No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6840.11981334342287804No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5900.10334776698757023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14950.048.7820051
GGTATCA6000.039.2091941
TCAACGC18000.039.164554
ATCAACG18500.038.106053
CAACGCA18750.037.5979655
AACGCAG19200.036.9615446
GTACATG30800.034.524461
TACATGG31650.033.44862
ACATGGG31600.033.3146553
GGTACCT4950.031.331648
TATCAAC23750.030.1126632
CGCAGAG23950.029.8271988
ACGCAGA23950.029.6309667
CATGGGG17600.029.106384
ATGGGCG650.00615323228.9215165
GGGTACC5200.028.9215167
TAGGTAT2151.8189894E-1228.417075
TAATACC1205.8388814E-627.4151864
GCAGAGT26550.026.9062659
GTTGACG350.00883455226.85804436-37
Produced by FastQC (version 0.11.2)