FastQCFastQC Report
Sun 14 Apr 2019
SRR6577016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818955
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA32070.39159660787222744No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22050.2692455629430188No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19990.24409155570208374No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC13550.1654547563663449No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC13100.15995994895934454No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10640.12992166846774242No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT10040.12259525859174192No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA8710.10635505003327411No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8620.10525608855187403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3950.055.9464721
GTATCAA17850.054.7894671
TCAACGC20400.047.4595224
ATCAACG20800.046.5468333
CAACGCA21300.045.4541855
AACGCAG22100.043.5961236
TATCAAC24600.039.182372
ACGCAGA26650.036.329247
CTTAGGT6200.035.6280823
GTACATG34150.034.4208221
CGCAGAG28100.034.2873468
TACATGG34400.034.1706732
TTAGGTA6350.034.0463374
GTCTTAG7050.034.0136341
GTACCGT702.1847979E-433.5705346
ACATGGG35250.033.065793
TAGGTAT6550.033.0067565
GCAGAGT30450.031.6411959
GTGCAAG4050.031.3458961
AGGTATA6750.031.3324996
Produced by FastQC (version 0.11.2)