FastQCFastQC Report
Sun 14 Apr 2019
SRR6577017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731644
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA31580.43163068377516933No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22720.3105335381688362No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17600.2405541492857182No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC12590.1720782238356359No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC12390.16934465395738912No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT9290.12697432084456375No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9290.12697432084456375No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8090.11057290157508297No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA8010.10947947362378424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3200.064.616441
GTATCAA14850.053.4811361
ATCAACG17400.044.5569763
TCAACGC17400.044.5569764
CAACGCA18150.042.715785
AACGCAG18650.041.822536
TATCAAC19100.040.596722
AAAACCG500.001703872937.5898865
GGTAATC3250.033.252598
ACGCAGA24300.032.098367
CGCAGAG24500.031.6445478
TTAGGTA7000.030.8774034
TAGGTAT6750.030.6287985
GTGCAAG4350.030.248871
TACATGG28800.029.8606322
GTACATG29400.029.5709151
CTTAGGT7050.029.3254453
ACATGGG29550.029.257783
AAGGGTA3700.029.2083595
AGGTATA7150.028.9152976