FastQCFastQC Report
Sun 14 Apr 2019
SRR6577017_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577017_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731644
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA31010.423840009622166No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC21130.2888016576367741No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18830.257365604036936No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11900.16264740775568445No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11240.15362662715747002No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8750.11959368217329738No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG8720.11918364669156038No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA7730.10565247579423873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2800.055.4932631
GTATCAA13550.054.9037671
GGTAATC2500.043.262098
TCAACGC16700.042.7710384
ATCAACG16950.042.14023
CAACGCA17150.041.648775
AACGCAG17750.040.5084346
TATCAAC20000.035.9487762
TACATGG27800.034.8213122
GTACATG28550.034.633591
AAGGGTA3150.034.311525
GTACGGG550.00271867234.1759033
ACGCAGA22000.032.6807067
ACATGGG29750.032.065043
CGCAGAG22700.031.9017498
AGGGTAA2950.031.861076
GTGCAAG4150.030.6337411
CATGGGG19200.029.6146684
CAAGGGT3850.029.2936334
ATACCGA650.00615587328.9200486
Produced by FastQC (version 0.11.2)