FastQCFastQC Report
Sun 14 Apr 2019
SRR6577020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731543
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27340.37373059410041515No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16780.2293781773593623No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16280.22254330914245643No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11120.15200746914398744No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10470.14312214046200974No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8590.11742303596644352No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8360.1142789965866668No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8310.11359550976497623No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7930.10840100992012772No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7850.10730743100542278No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7720.10553036526902726No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7670.10484687844733666No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7580.10361660216829359No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7490.10238632588925053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2500.062.045961
GTATCAA15650.057.6668781
TCAACGC17800.048.582294
CAACGCA18200.048.0310135
ATCAACG18500.046.7440383
AACGCAG19350.044.933576
TATCAAC22400.039.660062
GGTACCT3200.039.6546368
ACGCAGA23750.036.8069237
GGTAATC3450.035.4188468
ACCTAAG9450.035.315561
AAGGGTA3350.035.07325
CGCAGAG25100.034.6400228
TACATGG31250.034.444912
GTACATG31200.034.3494531
CTAACGC550.00271690834.1804283
ACATGGG32300.032.884113
CTTAGGT4550.032.0206763
GCAGAGT27350.031.7902959
CTAAGAC10700.031.6248783
Produced by FastQC (version 0.11.2)