FastQCFastQC Report
Sun 14 Apr 2019
SRR6577021_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577021_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670356
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28080.4188819075237635No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15740.23480061340541442No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14600.21779472399739838No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10390.15499227276253216No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC9500.14171574506680032No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7780.1160577364862849No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7610.11352177052193163No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7150.10665974497132867No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7090.10576469816038045No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7080.10561552369188908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.10501882581792361No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6800.10143863857413075No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6780.10114028963714802No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6780.10114028963714802No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6740.10054359176318253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2900.063.33241
GTATCAA13550.058.041221
TCAACGC16450.047.7056164
ATCAACG16650.047.1325763
CAACGCA16950.046.0211335
AACGCAG17900.044.1037186
TATCAAC19800.039.396882
CGCAGAG21250.036.9573258
ACGCAGA21300.036.8430677
GTGCTAC801.1422946E-535.3199921
GTACATG28750.035.0538141
ACATGGG28000.034.7401163
TACATGG28550.034.5646482
TTAGTAC550.002718625434.175613
ATTATCC8550.032.9764633
GCAGAGT23850.032.9284329
TTAGGTA5400.032.1978534
TAGGTAT5400.032.1978535
TTAAGAC2200.032.039633
CTTAGGT5600.031.887063
Produced by FastQC (version 0.11.2)