FastQCFastQC Report
Sun 14 Apr 2019
SRR6577022_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577022_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779398
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA41720.5352849250318836No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC26260.33692670496973304No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC24760.3176810820659022No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC16230.20823763981945037No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC15950.20464512354406864No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT12570.161278319934103No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11680.14985925034449665No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA10730.13767035583873707No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT9210.11816812462952177No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT9130.11714169140798411No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG8520.10931513809375953No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8220.10546601351299335No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8150.10456788444414791No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.10443958029145571No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT8130.10431127613876351No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG8050.10328484291722585No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA7950.10200180139030379No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT7870.10097536816876615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3750.061.591791
GTATCAA17800.054.2865031
ATCAACG20000.047.711613
TCAACGC20000.047.711614
CAACGCA20800.046.1025435
TATCAAC21300.045.241012
AACGCAG21650.044.726756
GGTAATC2700.040.0348788
TTAGGTA9550.037.4083254
TAGGTAT9500.037.110415
CTTAGGT9650.036.0464443
GTACATG34400.036.0375561
GGTATAG9750.035.6721577
GTCTTAG10150.035.2943761
ACGCAGA26700.035.2063527
AGGTATA9950.034.959626
CGCAGAG27000.034.812948
TACATGG35400.034.2589872
ACATGGG35800.033.4822923
GTATAGT12250.032.994148
Produced by FastQC (version 0.11.2)