FastQCFastQC Report
Sun 14 Apr 2019
SRR6577028_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577028_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568361
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22560.3969308238953763No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC15630.2750012755977275No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14560.25617521258495923No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC8550.15043255958800833No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8290.14585800222041975No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7340.12914327337730774No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7220.12703193920765146No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT6940.12210549281178687No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT6200.10908559876557329No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT6040.10627048653936494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11550.056.2399141
GGTATCA2550.053.5310021
ATCAACG12150.049.892413
TCAACGC12150.049.892414
CAACGCA12750.047.544535
AACGCAG13100.046.2742586
TATCAAC14850.041.4539452
GTACATG21450.037.3051831
ACGCAGA16400.036.9662257
CGCAGAG16850.036.841658
TACATGG22000.035.6709632
ACATGGG22850.034.5496863
TAGGTAT5300.033.6921545
TTAGGTA5350.033.3772744
GCAGAGT19200.032.5773289
CTTAGGT5500.030.7581943
GTATAGG1102.9305884E-629.953871
AGGTATA5600.029.3698026
GGTATAG5650.029.1124537
GTACTAA650.006106782728.966381
Produced by FastQC (version 0.11.2)