FastQCFastQC Report
Sun 14 Apr 2019
SRR6577029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822082
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26860.3267313966246676No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18600.22625480183242058No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14170.17236723343899998No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC11470.13952379446332605No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9960.12115579711026393No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9610.11689831428008399No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8890.10814006388657092No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8810.10716692495395835No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8680.10558557418846296No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8610.10473407762242697No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8600.1046124352558504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4250.060.839911
GTATCAA16900.056.1926421
TCAACGC19600.046.998364
ATCAACG20350.045.2662283
CAACGCA20600.044.488745
AACGCAG21050.043.537676
TATCAAC23500.039.2105522
GTCTTAG6850.037.7473871
TAGGTAT5900.035.049625
ACGCAGA26350.034.7805677
CGCAGAG26750.034.2604838
TTAGGTA6050.034.1806264
AGGTATA5900.033.456466
CTTAGGT6250.032.3348733
ACCTAAG8950.031.5168631
CGTAACA300.0041622131.33223588-89
GCAGAGT30650.030.2077549
GGTATAG6650.029.6831727
CTAAGAC10450.029.683173
GTACATG34250.029.3743321