FastQCFastQC Report
Sun 14 Apr 2019
SRR6577029_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577029_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822082
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26510.32247391379448764No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17610.21421220754134015No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13550.16482540671125265No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10440.12699463070593933No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9320.11337068564936344No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9240.11239754671675088No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT8700.1058288589216161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16500.060.8289151
GGTATCA4400.057.8303071
TCAACGC19350.050.525694
ATCAACG19350.050.2827763
CAACGCA19950.049.0061235
AACGCAG20900.047.2283676
TATCAAC22300.044.2633552
ACGCAGA27450.035.4409267
CGCAGAG27600.035.2504588
GCAGAGT29500.032.9800879
ACCTAAG9150.027.8091091
GTACATG32450.027.299681
TACATGG32550.027.0035272
CTAAGAC9850.026.7227823
CCTAATA1659.304313E-825.6382542
GTCTTAG8100.025.5965881
TAAGACA12700.025.5373174
TTAGGTA7200.025.4602134
TAGGTAT7050.025.3352015
ACATGGG34250.025.2514973
Produced by FastQC (version 0.11.2)