FastQCFastQC Report
Sun 14 Apr 2019
SRR6577030_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577030_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735195
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25950.352967580029788No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17620.23966430674854972No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12770.17369541414182632No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10570.1437713803820755No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8520.11588762165139861No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7980.10854263154673251No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7630.10378198981222668No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT7590.1032379164711403No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7360.10010949475989363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14450.057.980451
TCAACGC16100.051.6615334
ATCAACG16600.050.1054653
GGTATCA3750.048.9511341
CAACGCA17050.048.507425
AACGCAG17550.046.8576856
TATCAAC19300.043.1076162
ACGCAGA21200.038.568557
CGCAGAG21500.038.2723738
GCAGAGT24250.034.126119
GTACATG28550.032.1482931
TACATGG28900.032.041032
ACCTAAG8750.030.1237751
ACATGGG30150.030.0808373
CTAAGAC9700.029.5513973
TATAGGG804.776635E-429.377722
AGAGTAC21400.028.23418210-11
GGTACCT3400.027.6590278
ATAGTAC856.820888E-427.6420973
TAAGACA11600.027.1416824