FastQCFastQC Report
Sun 14 Apr 2019
SRR6577042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595801
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11270.18915711789674738No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11260.18898927662088516No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10450.1753941332760435No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9800.16448445034499773No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9350.15693159293119682No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9200.1544139737932632No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8330.13981178279324807No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8130.13645495727600324No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8110.13611927472427873No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7970.13376949686220735No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7960.1336016555863451No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7790.130748353896687No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7720.12957346496565128No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7120.11950298841391672No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7090.11899946458632998No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6660.11178228972425358No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT6540.10976819441390664No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6540.10976819441390664No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC6470.10859330548287095No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6200.10406159103459041No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6150.10322238465527919No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC6040.10137613062079452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10450.059.8414761
ATCAACG13550.045.4377373
CAACGCA14000.043.305835
AACGCAG15050.041.221346
TAATAGT802.4564906E-741.1237534
GTATAAG1500.037.614641
TCAACGC16500.037.314024
CGGGCGT1353.45608E-1034.813766
ACGCAGA17550.034.278167
CGCAGAG17650.034.083958
GGTATCA2950.033.4706541
GGGTAGC1001.4053712E-632.912811
TATCAAC19250.032.729622
TACATGG22650.032.175882
ACATGGG22500.032.167913
GTACATG22600.032.039021
GTATATA600.00415146831.3455351
GCAGAGT19450.030.9296539
CATGGGG15400.029.2978124
TGATCGC1652.9594958E-928.4839849
Produced by FastQC (version 0.11.2)