FastQCFastQC Report
Sun 14 Apr 2019
SRR6577042_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6577042_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595801
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11580.19436019744847693No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10320.17321219668983437No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9440.158442164413957No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8810.14786816403463574No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8630.14484702106911537No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8530.14316860831049294No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8350.14014746534497258No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7690.12906994113806455No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG7690.12906994113806455No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7470.1253774330690952No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7450.12504175051737074No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7390.12403470286219728No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC7150.12000651224150347No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7050.11832809948288103No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6770.11362854375873825No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6750.11329286120701375No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6730.11295717865528927No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT6350.10657921017252406No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6180.10372590848286593No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6100.10238317827596798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9100.054.8042561
CAACGCA12050.040.172235
ATCAACG12450.038.8815543
GTACTAG1900.034.667641
AACGCAG14350.034.3884966
TCAACGC14300.034.180084
GGTATCA3150.032.8595771
GTACATG20250.031.365961
TATCGTC753.2746396E-431.331747
ACGCAGA15900.030.4449927
TACATGG20350.030.2539882
AAGTTAA2650.030.15194194
CCTTTAT1102.9413259E-629.9402311
ACATGGG20800.029.3735073
CGCAGAG16500.029.33798
TATCAAC17600.028.3053762
ACCTAAG4400.027.8016431
CGATAAT1800.027.4175766-67
GCAGAGT17850.027.382369
ACCAGTA1606.907794E-826.4361532
Produced by FastQC (version 0.11.2)