FastQCFastQC Report
Thu 8 Jun 2017
Stat5_FH.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameStat5_FH.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26317787
Sequences flagged as poor quality0
Sequence length36
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG565020.21469130364190578Illumina Paired End PCR Primer 2 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA67200.028.2294061
ATCGGAA69300.026.7020822
ATGCCGA68800.026.61504425
GAGCGGT70300.026.1299829
AGCGGTT70850.026.01177810
CGAGACC66650.025.95213129
GCGGTTC71150.025.77653311
AAGAGCG72100.025.7065737
CCGAGAC67800.025.554628
GAGACCG68050.025.50617630
CGGTTCA72450.025.39683712
AATGCCG73700.024.92656724
GCCGAGA73800.024.91806427
AGAGCGG75150.024.6434148
TCGGAAG75650.024.5200563
TGCCGAG75550.024.2794726
CGGAAGA77250.024.0509914
GAATGCC101600.018.47848323
GGAATGC102500.018.16988222
GTTCAGC105600.017.65446314
Produced by FastQC (version 0.11.2)