FastQCFastQC Report
Thu 8 Jun 2017
Stat5_FL.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameStat5_FL.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41434745
Sequences flagged as poor quality0
Sequence length36
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA258750.027.80031612
CGAGACC252300.027.5067429
AGCGGTT263300.027.37510910
GAGACCG255450.027.19462630
CCGAGAC255850.027.17163528
GAGCGGT267400.027.0147069
GCGGTTC268500.026.829911
ATGCCGA268550.026.61196725
AAGAGCG275300.026.543937
AATGCCG272800.026.2027824
GCCGAGA272200.026.06918127
AGAGCGG280500.026.0092898
TGCCGAG283750.025.17133526
ATCGGAA298450.024.9698492
GATCGGA298350.024.9245241
CGGAAGA299300.024.872894
TCGGAAG299900.024.8570213
GGAATGC307700.023.4949622
GTTCAGC310200.023.28118514
GAATGCC321050.022.49172423
Produced by FastQC (version 0.11.2)