FastQCFastQC Report
Thu 8 Jun 2017
Stat5_MH.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameStat5_MH.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57479355
Sequences flagged as poor quality0
Sequence length35
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA30400.018.409911
TCGTATG31300.017.74143613
CGTATGC32100.017.11860814
CTCGTAT32950.016.85304612
ATGCCGT36000.014.90167217
TGCCGTC36250.014.75899618
GCCGTCT37250.014.63520719
CCGTCTT38300.014.46103220
CGTCTTC43600.012.63715121
TATGCCG47650.011.8060516
AGCTCGT49300.011.49910710
GAGCTCG52650.010.7399169
GTATGCC75500.07.796759615
TACGGCG4556.1765604E-86.6918826
CGGCGAC8550.06.10488
CGGAAGA107550.05.5946044
TCGGAAG123300.05.491893329
ATCGGAA132650.05.006498328
Produced by FastQC (version 0.11.2)