FastQCFastQC Report
Mon 20 Nov 2017
JG-TeamD-Ec-yciT-FLAG_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJG-TeamD-Ec-yciT-FLAG_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14293413
Sequences flagged as poor quality0
Sequence length62-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC387180.27088002004839573TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG86800.044.2305970
TCTCGTA66100.042.36195841
TCGTATG68750.042.14591243
ATCTCGT66750.041.581940
CTTGAAA77300.036.84973560
GTCTTCT78800.035.88860352
ACATCTC77750.035.78627438
TACATCT77900.035.62807537
GCTTGAA82500.034.6964459
CGTATGC89500.032.49124544
GCTACAT87900.032.48828535
CTACATC87600.032.00102636
CATCTCG88000.031.97369239
GTATGCC90000.031.96289645
CTCGTAT91500.031.21082142
ACGGCTA93800.030.70467832
CCGTCTT96500.029.91432450
GGCTACA96800.029.78901934
GCCGTCT98100.029.35541549
TGCTTGA99400.028.65989358
Produced by FastQC (version 0.11.5)