FastQCFastQC Report
Mon 20 Nov 2017
JG-TeamE-Ec-uvrY-FLAG_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJG-TeamE-Ec-uvrY-FLAG_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15139051
Sequences flagged as poor quality0
Sequence length64-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT301010.19883016445350504TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA52300.043.62592743
TCGTATG63300.037.2536245
CTCGTAT64200.036.08126444
CTTGAAA67000.034.1559562
ATCTCGT69550.033.05573342
GTATCTC72050.032.1002740
TCCGTAT79200.030.82589737
GTCTTCT74050.030.25036254
CGTATCT78150.029.50450739
GCTTGAA78250.029.06737761
CGTATGC85750.027.70314846
TCACAGT90100.027.10142530
TTCCGTA91050.026.77629336
TATCTCG88550.026.31653841
CCGTATC96400.024.85684438
CCGTCTT93200.024.66921452
GTTCCGT98450.024.66117535
GTATGCC98300.024.37760247
TGCTTGA93700.024.08877260
AGTCACA103200.023.75920328
Produced by FastQC (version 0.11.5)