FastQCFastQC Report
Wed 23 Nov 2016
WT_control_B_noAb_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWT_control_B_noAb_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25848784
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT836130.3234697616723479TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA133950.046.7486243
TCGTATG140350.045.55895645
CTTGAAA150300.042.79271762
ATCTCGT150250.041.19098342
GTCTTCT159050.040.1079754
ACATCTC163700.038.1403140
GCTTGAA169100.038.11980461
CTCGTAT167550.037.74744844
CGTATGC175950.036.71681646
CACATCT176150.035.4227439
GTATGCC185600.034.788947
CATCTCG186400.033.3483241
TGCTTGA194800.033.10812460
CCGTCTT198000.032.5873352
GCCGTCT203500.031.70690251
CACGTCC210500.031.06658231
GTCCGCA217150.030.15975634
CGCACAT212200.030.12700537
CTGCTTG215200.029.87205359
TCACGTC229450.028.49989130
Produced by FastQC (version 0.11.5)