FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-ACAGTG_ACAGTG_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-ACAGTG_ACAGTG_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16463860
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA18700.028.3859651
TCGTATG19400.028.00963443
CGTATGC19850.027.1536644
CTCGTAT19600.025.478942
ACACGTC25650.021.27031313
ATCGGAA25300.021.1297972
ATCTCGT24850.019.29927440
TCTCGTA25550.018.5983241
GATCTCG26950.017.71380639
CGTCTGA32800.016.83485816
CACGTCT34400.016.371614
CACACGT34750.016.20670512
ACGTCTG35100.015.73171815
TCGGAAG38550.015.1798273
CGGAAGA39450.014.7777564
CTCGGTT17650.013.1030641
GCACACG45100.012.38987111
AGTGATC41550.012.23067836
CTCCGTT21850.011.9956491
CTCGCAA10000.011.2330681
Produced by FastQC (version 0.11.2)