FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-ACAGTG_ACAGTG_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-ACAGTG_ACAGTG_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16463860
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT17750.030.48121311
CGTCGTG19750.028.06292312
AGCGTCG20950.025.61540810
CGTGTAG21250.025.56365615
TCGTGTA21000.025.44906814
GATCGGA21500.025.1630421
GTCGCCG21750.024.07806844
GATCTCG22700.023.16930234
GAGCGTC24850.022.3916959
AGAGCGT25950.021.5266958
GTGGTCG24900.020.85879341
ATCGGAA25900.020.6335662
GGTCGCC25550.020.50022543
TGGTCGC26000.020.48417942
ATCTCGG26400.020.17246435
AAGAGCG28800.019.2436037
GTCGTGT28900.019.02574213
TCTCGGT30300.017.28575936
GTAGATC32850.016.67917431
TCGGAAG34100.015.8653093
Produced by FastQC (version 0.11.2)