FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-ACTTGA_ACTTGA_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-ACTTGA_ACTTGA_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18221856
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC20550.026.65683744
TCGTATG20950.026.35724643
GATCGGA21050.026.056331
CTCGTAT22000.023.99938242
ACACGTC28050.020.15623513
ATCGGAA27350.019.8677522
AATCTCG26400.018.66613639
TCTCGTA26550.018.56067741
ATCTCGT28100.018.08489440
CGTCTGA33650.016.73647716
ACGTCTG36350.015.85645115
TCGGAAG38800.015.7056963
CACACGT38950.014.74151712
CGGAAGA41600.014.4370494
CACGTCT39250.014.40464714
CTCGGTT19900.012.3974091
GTCCGTT9800.011.9128531
CTCCGTA15000.011.307471
CTCCGTT27400.011.1745561
CTCGGAT23850.011.0830391
Produced by FastQC (version 0.11.2)