FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-ACTTGA_ACTTGA_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-ACTTGA_ACTTGA_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18221856
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG20950.026.6657612
TCGTGTA22100.025.17734114
CGTGTAG22050.025.03495415
AGCGTCG24650.022.93080710
GCGTCGT24100.022.90655511
GATCGGA24350.022.5789431
GTCGCCG23700.022.56158644
GATCTCG26000.021.2443934
ATCTCGG26200.021.16627135
GGTCGCC25250.020.7436243
GTGGTCG26350.020.21196241
ATCGGAA26900.020.1933542
GAGCGTC28350.020.1705369
TGGTCGC29050.018.40915742
AGAGCGT31200.018.256848
GTCGTGT32050.017.56775513
AAGAGCG32850.017.0719877
TCTCGGT33650.016.28420436
GTAGATC38150.015.39997931
TCGGAAG38300.014.7570013
Produced by FastQC (version 0.11.2)