FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-ATGTCA_ATGTCA_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-ATGTCA_ATGTCA_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20113738
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA13950.015.4736191
CTCGTAT13200.013.66686944
CTCGGTT21000.013.3206221
CTCCGTT26450.012.2414191
CTCGCGT4650.011.8420571
TCGAAAT14950.011.7721642
TCCGATT26400.011.749592
GTCCGAT24250.011.6262131
TCGGGTT16550.011.564552
CGCGTAT2108.020508E-611.5375471
CGCGACA15500.010.92881442
ACCGCGA16150.010.48890240
GTCGAAA18250.010.3794821
ATCGGAA20000.010.3396392
CCGATTT26550.010.2745943
ACACGTC21200.010.27322713
CGGGGTT23600.010.2664611
CGAAATA24450.010.2573113
AATCTCG16200.010.18493141
CCGCGAC16950.010.12364141
Produced by FastQC (version 0.11.2)