FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-ATGTCA_ATGTCA_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-ATGTCA_ATGTCA_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20113738
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG13800.014.66980144
AGCGTCG14400.014.66250610
CGTCGTG15250.014.27795212
CGTGTAG14650.014.26166915
GCGTCGT14550.014.05786611
TCGTGTA15950.013.09927614
GATCGGA16450.012.5666121
TGGTCGC17750.012.27457142
GTGGTCG19000.011.00371941
GATCTCG19400.010.7763334
ATCTCGG20900.010.31881535
GGTCGCC19600.010.21756243
GAGCGTC21850.09.8644059
AGAGCGT26300.09.1147868
CGTCGAT9600.08.93460917
ATCGGAA22400.08.9340762
GTCGTGT24750.08.8863913
TCGTCGA10400.08.45880116
CGGTACG3809.0351095E-68.10723726
TCTCGGT27100.07.958191436
Produced by FastQC (version 0.11.2)