FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-CAGATC_CAGATC_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-CAGATC_CAGATC_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19502556
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC24800.029.18561444
GATCGGA25150.029.1639731
TCGTATG25400.028.58192343
CTCGTAT26400.026.74936542
ATCGGAA32600.022.4716852
TCTCGTA31000.021.9285141
ACACGTC34550.021.2670613
ATCTCGT33600.020.75545940
CGTCTGA37700.019.89857916
ACGTCTG40450.018.98086215
CACGTCT42800.017.68168614
TCGGAAG45050.017.1892493
CATCTCG41400.016.89814839
CACACGT43950.016.81858312
CGGAAGA46850.016.6227424
GCACACG58700.013.04217911
GATCATC55550.012.75213936
TCGGATT13800.011.9561462
GTCGGTT12850.011.8273221
ACCAGAT63250.011.721432
Produced by FastQC (version 0.11.2)