FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-CAGATC_CAGATC_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-CAGATC_CAGATC_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19502556
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT24950.029.0896511
CGTCGTG25550.028.23451212
TCGTGTA26950.026.8484314
GTCGCCG26150.026.2537844
AGCGTCG28100.026.06351910
CGTGTAG28100.025.82791715
GATCGGA28550.025.1114671
GATCTCG30600.023.51628534
GGTCGCC29450.023.4646843
GAGCGTC31500.023.3200159
GTGGTCG30050.023.21605341
TGGTCGC30700.023.08293742
AGAGCGT32500.023.0076248
ATCTCGG30850.022.9692935
ATCGGAA32350.022.0260072
TCTCGGT36200.020.12203236
GTCGTGT36950.019.76154913
AAGAGCG36850.019.5753847
CGGTGGT40750.017.93015539
TCGGAAG43350.016.7920173
Produced by FastQC (version 0.11.2)