FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-CCGTCC_CCGTCC_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-CCGTCC_CCGTCC_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15053837
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT15750.013.1461671
GTCCGTT9400.012.8880331
TCGAAAT12500.012.8475982
CTCGGAT20000.012.5552871
TCGGTTT12000.012.4662762
GTCCGAT18550.012.1117841
TCCGATT18750.011.8502962
CGAAATA16500.011.7329663
CTCGTAT7800.011.0134111
CTCCGGT19950.010.5993721
CTCCGTT19500.010.5050991
CCGATTT18700.010.4702613
CTCCGAA17450.010.3507121
GTCGGAT8100.010.33357051
GTCGGTT9600.010.3250721
CTCCTAT40150.010.2042061
TCGGGAT10800.010.1848672
TCGCATT8450.010.1535292
CCCGTAT6450.09.9035321
TCCGATA7400.09.8105042
Produced by FastQC (version 0.11.2)