FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-CCGTCC_CCGTCC_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-CCGTCC_CCGTCC_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15053837
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22000.030.2891831
TATGCCG21150.020.1725333
GTATGCC35700.016.8791832
ATGCCGT21950.016.631934
TGCCGTC22200.015.6518095
GCCGTCT21200.014.9378146
CCGTCTT21400.012.5378287
CGAAAAC12150.012.3083621
CGACGAT4550.011.6002531
CGAAAAA23800.010.9036691
CAAAGAT79000.010.8012071
CGCGAAT5300.010.78861
CTCAGAT81700.010.6326781
CGACGAA5400.010.5888121
CTAAAAT53850.010.5774671
CGAAGAC23200.010.5221051
CGAAAAT9650.010.4833031
CGACAAT7350.010.472451
CGGGAAT13950.010.4048861
CTCAAAT47000.010.3877791
Produced by FastQC (version 0.11.2)