FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-CGATGT_CGATGT_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-CGATGT_CGATGT_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16526149
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC15150.025.84876344
GATCGGA15750.024.6142431
TCGTATG16900.023.04102743
CTCGTAT17050.022.32226842
CGATGTA19000.020.26256634
TATCTCG17250.020.15025939
CACCGAT21050.019.02082431
TCACCGA21050.018.81180430
ATCGGAA20850.018.2535532
GTCACCG22650.017.9685729
ACCGATG22950.017.254432
CCGATGT23100.017.04712333
ACACGTC23800.016.9153713
ATCTCGT22000.016.09962840
TCTCGTA22600.015.8668941
CGTCTGA27000.015.56241516
ACGTCTG29600.013.9724815
TCGGAAG30300.013.9401033
CACGTCT30750.013.66455914
CACACGT30850.013.40633612
Produced by FastQC (version 0.11.2)