FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-CGATGT_CGATGT_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-CGATGT_CGATGT_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16526149
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG15200.025.17710512
GCGTCGT15500.024.83162711
TCGTGTA16950.022.31764614
AGCGTCG17800.021.870110
GTCGCCG17100.021.61794544
GATCGGA18050.021.3216861
GATCTCG19600.020.20987734
CGTGTAG19000.020.14119515
GAGCGTC20850.019.408989
GTGGTCG19850.019.18093141
ATCTCGG21650.018.29646135
GTCGTGT21250.018.00903313
AGAGCGT22600.017.6133278
GGTCGCC21050.017.56450743
TGGTCGC21850.017.52596342
ATCGGAA22000.017.493582
TCTCGGT24350.016.7198336
AAGAGCG26950.014.6886657
CGGTGGT29300.013.59561839
TCGGAAG30700.013.1091173
Produced by FastQC (version 0.11.2)