FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-CTTGTA_CTTGTA_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-CTTGTA_CTTGTA_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18906914
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC193460.10232235678440173TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA29300.033.077551
TCGTATG29650.031.68199243
CGTATGC30250.031.34523244
CTCGTAT30750.029.4756742
AATCTCG30900.027.83747739
ATCGGAA35950.026.9254552
ACACGTC36650.026.47121813
TCTCGTA35500.024.54021841
ATCTCGT37400.023.35234640
CGTCTGA42900.022.61468516
ACGTCTG45000.021.75488315
CACGTCT45150.021.58516114
CGGAAGA47650.021.4222154
CACACGT45500.021.4191212
TCGGAAG49900.020.632633
GCACACG56100.017.68572811
GTAATCT56000.015.71388437
TAATCTC63850.013.9197738
TGTAATC66050.013.5560536
TTGTAAT68550.013.54301735
Produced by FastQC (version 0.11.2)