FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-CTTGTA_CTTGTA_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-CTTGTA_CTTGTA_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18906914
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG197860.10464954777918808Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT27300.033.8361511
CGTCGTG28800.032.3032112
AGCGTCG30000.031.08414810
TCGTGTA31400.029.62693214
CGTGTAG31450.029.57983215
GTCGCCG31300.028.82424244
GATCGGA33250.028.639041
GTGGTCG32850.027.66852241
GAGCGTC35450.026.3052029
GATCTCG36200.025.77513534
GTCGTGT36450.025.76488113
GGTCGCC35650.025.68025443
ATCTCGG37200.025.02329635
TGGTCGC36250.025.01269142
AGAGCGT38250.024.4937528
TCTCGGT38850.023.84768736
ATCGGAA40700.023.1808682
AAGAGCG43500.021.739847
TCGGAAG46650.020.6485353
CGGTGGT46200.020.05453339
Produced by FastQC (version 0.11.2)