FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-GATCAG_GATCAG_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-GATCAG_GATCAG_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17881423
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT23300.032.09364311
CGTCGTG25500.028.89377612
CGTGTAG25650.028.46613915
AGCGTCG27100.027.91805810
TCGTGTA27400.026.88884214
GATCGGA28300.026.3433911
GTCGCCG28400.026.11058444
GAGCGTC31050.024.9330069
GTGGTCG30500.023.88441341
GATCTCG32150.023.2726334
GTCGTGT32050.022.98880813
ATCGGAA33100.022.5896422
ATCTCGG33450.022.4343335
TCTCGGT33500.022.2697136
AGAGCGT34750.022.1506378
GGTCGCC33250.022.04114343
TGGTCGC33800.021.74784342
CGGTGGT36400.020.91997539
AAGAGCG36850.020.5898597
GTAGATC41050.018.38635631
Produced by FastQC (version 0.11.2)