FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-GCCAAT_GCCAAT_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-GCCAAT_GCCAAT_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23014796
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA29700.030.254931
TCGTATG29500.029.97885743
CGTATGC29800.029.67782844
GTCACGC31250.028.72242729
CTCGTAT30600.028.46982242
TATCTCG29600.028.01951639
CGCCAAT33550.026.4255233
ATCGGAA37600.023.87172
ACGCCAA37900.023.74078832
TCACGCC37950.023.70950730
AGTCACG39450.023.58872228
ACACGTC41600.021.8407613
TCTCGTA38500.021.42799841
ATCTCGT41200.020.3975140
CACGCCA48150.018.9610731
CGTCTGA49450.018.6405516
ACGTCTG52950.017.65769415
TCGGAAG54850.017.527333
CACACGT54400.017.18703812
CACGTCT53800.017.1742614
Produced by FastQC (version 0.11.2)