FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-GGCTAC_GGCTAC_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-GGCTAC_GGCTAC_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14383790
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT22750.032.2016442
TCGTATG23200.031.9563443
GATCGGA24450.031.7047621
CGTATGC23200.031.67208344
ACGGCTA24650.030.87968332
CGGCTAC28400.026.80225833
ACACGTC30400.025.83480813
ATCGGAA29950.025.7822532
TCTCGTA28450.024.51275441
TCACGGC31400.024.4517230
AGTCACG31800.024.21316328
ATCTCGT29900.023.39758740
GTCACGG34300.022.76920529
CACGTCT34250.022.73805414
CACACGT34850.022.5359612
CGTCTGA34850.021.96782516
ACGTCTG36450.021.54672415
TCGGAAG38400.021.0827483
CGGAAGA37900.021.0126084
CACGGCT37550.020.62273831
Produced by FastQC (version 0.11.2)