FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-GTCCGC_GTCCGC_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-GTCCGC_GTCCGC_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16758721
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG9300.022.47251932
GATCGGA12600.016.6062321
CGTCCGC13000.016.2457233
CTCGTAT13550.014.44992744
TCGAAAT8600.013.0461642
CTCCGTT21150.012.8089271
CTCGGTT15250.012.7095691
TCCGATT15850.012.0753872
CTCCGAT18750.011.9816571
CTCGCAT10200.011.8762831
ATCGGAA18150.011.7572622
TCTCGTA16650.011.3630643
CTCGGAT20200.011.3396581
GTCACGT19600.011.11194929
AGTCACG19350.011.02812928
CGCGACA15000.010.99970642
CGCACAT17400.010.99970637
TCACGTC19900.010.94443130
CTCGGTA13600.010.8506031
GTCGAAA10400.010.8007731
Produced by FastQC (version 0.11.2)