FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-GTCCGC_GTCCGC_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-GTCCGC_GTCCGC_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16758721
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG12550.016.12260210
GCGTCGT12450.015.89879511
GTCGCCG13150.015.56169644
CGTGTAG13850.015.24406715
CGTCGTG13550.014.77056312
GGTCGCC14550.014.36959743
TCGTGTA14850.013.92133214
GATCGGA15350.013.4673451
TGGTCGC16800.012.707252542
GATCTCG17250.012.62960934
GTGGTCG16750.012.48239741
ATCTCGG16950.012.33382335
GAGCGTC17200.012.1474089
ATCGGAA17850.011.4579932
TCTCGGT19900.010.94803836
AGAGCGT21650.010.259748
GTCGTGT23800.08.8713413
AAGAGCG25000.08.6210277
ATCCGCG5202.3810571E-98.4582936
GTCGATT6159.458745E-118.2246091
Produced by FastQC (version 0.11.2)