FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-GTGAAA_GTGAAA_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-GTGAAA_GTGAAA_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23514859
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328050.13950753436369742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA14250.014.6846011
CGAAATA25950.013.8184513
CTCGTAT15650.012.93291744
TCGAAAT16200.012.6292352
CTCGGTT25850.012.5259351
CTCGCAT15600.012.2842331
CTCCGTT31700.011.9515051
AACGATC14200.011.929255538
GTCCGAT28550.011.8042581
ACCGCGA21650.011.58398240
TCCGATT26000.011.422722
CGCGACA22900.011.33593942
AAACGAT16700.011.0655737
CTCCGGT26400.011.0134511
CTCCGAT24150.010.9450441
GTCGAAA17750.010.9203791
TCGGATT13150.010.8741862
GAAACGA19300.010.82872336
CTCGGGA47600.010.73581
TCGGTTT19050.010.6243392
Produced by FastQC (version 0.11.2)