FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-GTGAAA_GTGAAA_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-GTGAAA_GTGAAA_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23514859
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17700.016.1522161
GCGTCGT14600.014.16041111
GTCGCCG15750.013.13292744
TCGTGTA16850.012.13853714
CGTCGTG16500.011.86336312
TGGTCGC20450.011.29976442
CGTGTAG18000.011.11865315
AGCGTCG20100.010.61393710
GATCGGA20500.010.2986291
GGTCGCC20200.010.13209843
TATGCCG17400.09.8584633
GATCTCG21650.09.75795834
GAGCGTC22100.09.6533589
ATCTCGG23550.09.1576435
GTGGTCG23000.08.8987441
AGAGCGT26800.08.69862658
ATCGGAA26200.08.0581232
TCTCGGT29200.07.61190636
GTCGTGT30450.07.29517713
CGGACAT25350.07.1137391
Produced by FastQC (version 0.11.2)