FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-TGACCA_TGACCA_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-TGACCA_TGACCA_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26866376
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA39800.031.046681
CGTATGC39950.029.90231944
TCGTATG40250.029.62407143
CTCGTAT39850.028.81736842
ATCGGAA50150.024.7410352
AATCTCG44350.023.9588339
ACACGTC53050.023.05680513
ATCTCGT47500.022.46261440
TCTCGTA47450.022.1617441
CGTCTGA58200.020.97875416
TCGGAAG62700.020.9467413
ACGTCTG60750.020.5327215
CACGTCT62400.019.77825514
CGGAAGA66850.019.6463834
CACACGT65100.019.3972712
GACCAAT64800.017.41614735
GCACACG78950.016.21737511
ACCAATC73100.014.80672836
ACTGACC86850.014.46358732
TCGACCG8250.012.53302927
Produced by FastQC (version 0.11.2)