FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-TGACCA_TGACCA_BC8TK0ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-TGACCA_TGACCA_BC8TK0ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26866376
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT37050.032.5301411
CGTCGTG38200.031.49354412
AGCGTCG40100.030.11074610
GTCGCCG41050.028.785544
GATCGGA43000.028.435941
TCGTGTA42150.028.2801914
CGTGTAG42250.028.161215
GATCTCG45200.026.97234734
GAGCGTC48300.025.8182649
GTGGTCG46450.025.63247541
GGTCGCC46700.025.25920143
ATCTCGG48000.025.03251535
TGGTCGC50450.024.08002342
AGAGCGT52400.024.007198
ATCGGAA50750.024.0069262
GTCGTGT51100.023.50007813
TCTCGGT52350.023.16302736
AAGAGCG59250.020.9717677
GTAGATC60950.020.07288231
TCGGAAG65000.019.1498743
Produced by FastQC (version 0.11.2)