FastQCFastQC Report
Sat 11 Jun 2016
G123-pool-TTAGGC_TTAGGC_BC8TK0ANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG123-pool-TTAGGC_TTAGGC_BC8TK0ANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17606617
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA18250.027.6366271
CGTATGC19750.025.62009844
TCGTATG19650.025.52612743
CTCGTAT19950.024.81145542
ATCGGAA24650.020.259052
ACACGTC27600.018.97046913
TCTCGTA25750.018.36845641
CGTCTGA29700.017.9254116
ATCTCGT27400.017.90464440
ACGTCTG31100.017.40142815
CACGTCT34450.015.51769614
TCGGAAG36450.015.3301493
CACACGT35650.014.99536112
CATCTCG33450.014.79782239
CGGAAGA37800.014.7826444
CTCGGTT17550.013.1772451
CTCCGTT27900.013.1043761
GCACACG42800.012.64449611
GTCGGTT13050.012.4891721
CCCGTAT7250.012.1516271
Produced by FastQC (version 0.11.2)