FastQCFastQC Report
Fri 25 Nov 2016
G101M10.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG101M10.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences15509163
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC550290.35481605293593216TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG63600.038.15913439
TCGTATG66050.036.90049443
CGTATGC67650.036.0927944
GATCGGA69050.035.8462521
TAGCTTA69750.035.36420434
CTCGTAT70950.034.35260442
ACACGTC73350.033.88825613
ACGTCTG73450.033.8416815
CGTCTGA73350.033.7678616
TCTCGTA76100.032.5224741
ATCGGAA76800.032.114362
GCTTATC76700.031.98742136
ACTAGCT77400.031.83965732
CACGTCT78050.031.81898314
CACACGT81000.030.79632612
TCACTAG79950.030.63049530
ATCTCGT80650.030.44579540
CACTAGC82400.029.85355631
CTAGCTT84400.029.1470733
AGCTTAT87750.028.41055135
Produced by FastQC (version 0.11.2)