FastQCFastQC Report
Fri 25 Nov 2016
G101M11.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG101M11.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences14145824
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC430130.3040685364104629TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA48100.038.01352332
GATCGGA52850.035.2601171
TCGTATG52050.034.78157443
CTCGTAT52500.034.4003742
CGGCTAC53500.034.17700633
CGTCTGA56050.033.31978216
ACACGTC57100.032.82369213
ACGTCTG57500.032.55605715
AGTCACG56950.032.18169428
CGTATGC56600.032.06325544
CACGTCT60100.031.25755914
TCTCGTA59950.030.12852141
CACACGT62800.029.94955612
GTCACGG62000.029.5964529
ATCGGAA63200.029.4857142
TCACGGC62750.029.20764730
GCTACAT64650.028.38459835
CACGGCT66800.027.53602431
ATCTCGT66350.027.39029740
CGGAAGA73750.025.981894
Produced by FastQC (version 0.11.2)