FastQCFastQC Report
Fri 25 Nov 2016
G101M3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG101M3.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences10280365
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC412070.4008320716239161TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA50200.038.962841
CGCCAAT50550.038.3480433
ACGCCAA50800.038.07325432
TCGTATG51750.037.66059543
CGTATGC51500.037.62985244
CTCGTAT52000.037.43778242
GTCACGC52400.037.11922529
TATCTCG53000.036.7809639
ACGTCTG53900.036.5664715
ACACGTC54300.036.17610513
ATCGGAA54300.035.9400332
CGTCTGA55400.035.6161116
AGTCACG57200.034.27358628
TCTCGTA57000.034.079441
CACGTCT59100.033.38633714
TCACGCC58550.033.37059830
CACACGT59850.032.8581812
ATCTCGT59500.032.76223840
CACGCCA60250.032.46616731
CGGAAGA63400.031.3345584
Produced by FastQC (version 0.11.2)