FastQCFastQC Report
Fri 25 Nov 2016
G101M5.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG101M5.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences9759798
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1254291.285159795315436TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA136550.041.74731
ATCGGAA137650.041.3657342
TCGTATG136900.041.13350743
CGTATGC137700.040.8146644
CGTCTGA139600.040.7791416
ACACGTC139900.040.7709413
ACGTCTG139950.040.75574515
CTCGTAT138650.040.5193542
TCTCGTA140500.039.92790241
CACGTCT143600.039.70450614
CACACGT144650.039.53875712
CGGAAGA147700.038.8601044
ATCTCGT144500.038.7194340
AGTGATC144850.038.6144536
GCACACG149400.038.38473511
GATCTCG146300.038.37957439
TCGGAAG150100.038.0790943
GTCACAC160250.035.2734329
GTGATCT160100.035.0325137
GAACTCC163800.034.83870721
Produced by FastQC (version 0.11.2)